Article
Melanoma metastases from patients with hereditary cutaneous malignant melanoma contain a high frequency of N-ras activating mutations.
Melanoma research - 1 Jun 1994
Platz A, Ringborg U, Brahme E M, Lagerlöf B
Abstract excerpt
Mutations in N-ras exon 2 codon 61 were studied in formalin-fixed human melanoma metastases. DNA fragments including codon 61 were amplified by polymerase chain reaction (PCR) and mutational analysis was performed by oligonucleotide hybridization (ODN), allele specific PCR and PCR combined with s...
Topics
- Alleles
- Base Sequence
- DNA Primers
- DNA, Neoplasm
- Electrophoresis, Agar Gel
- Exons
- Female
- Genes, ras
- Humans
- Lymphatic Metastasis
- Male
- Melanoma
- Molecular Sequence Data
- Neoplasm Metastasis
- Point Mutation
- Polymerase Chain Reaction
- Skin Neoplasms