Article
Characterization of thymic nurse-cell lymphocytes, using an improved procedure for nurse-cell isolation.
Developmental immunology - 1 Jan 1993
Lahoud M, Vremec D, Boyd R L, Shortman K
Abstract excerpt
Thymic nurse cells (TNC), multicellular complexes consisting of lymphoid cells enclosed within cortical epithelial cells, were isolated from mouse thymus by a modified procedure allowing immunofluorescent labeling and flow cytometric analysis of their lymphoid contents (TNC-L). Collagenase was the only protease used for tissue digestion, to ensure that surface antigen markers remained intact. Zonal unit-gravity...
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