Article
Dissociation protocols influence the phenotypes of lymphocyte and myeloid cell populations isolated from the neonatal lymph node.
Frontiers in immunology - 1 Jan 2024
DaMata Jarina P, Zelkoski Amanda E, Nhan Paula B, Ennis Katherine H E, Kim Ji Sung, Lu Zhongyan, Malloy Allison M W
Abstract excerpt
Frequencies and phenotypes of immune cells differ between neonates and adults in association with age-specific immune responses. Lymph nodes (LN) are critical tissue sites to quantify and define these differences. Advances in flow cytometry have enabled more multifaceted measurements of complex immune responses. Tissue processing can affect the immune cells under investigation that influence key findings. To...
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