Article
Engineering Fab' fragments for efficient F(ab)2 formation in Escherichia coli and for improved in vivo stability.
Journal of immunology (Baltimore, Md. : 1950) - 15 Dec 1993
Rodrigues M L, Snedecor B, Chen C, Wong W L, Garg S, Blank G S, Maneval D, Carter P
Abstract excerpt
We previously developed an efficient route to humanized F(ab')2 fragments by high level secretion of the Fab' arms from Escherichia coli followed by directed chemical coupling in vitro. Here the number and type of interchain linkages in F(ab')2 molecules has been modified to simplify their production and improve their serum stability. All F(ab')2 variants had comparable binding affinity for the p185HER2 Ag and...
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