Article
A functional assay for heterozygous mutations in the GTPase activating protein related domain of the neurofibromatosis type 1 gene.
Oncogene - 2 Mar 1995
Ishioka C, Ballester R, Engelstein M, Vidal M, Kassel J, The I, Bernards A, Gusella J F, Friend S H
Abstract excerpt
The GTPase-activating protein related domain of the human neurofibromatosis type 1 protein (NF1GRD) can down-regulate RAS in Saccharomyces cerevisiae. Using a technique termed the FASAY method, for Functional Analysis of Separated Alleles in Yeast, we designed a rapid method for detection of heterozygous NF1GRD loss-of-function mutations. In our method, PCR amplified NF1GRD cDNA is directly cloned into a...
Topics
- Base Sequence
- Cell Transformation, Neoplastic
- GTPase-Activating Proteins
- Genes, Neurofibromatosis 1
- Genes, ras
- Heterozygote
- Molecular Sequence Data
- Mutation
- Proteins
- Saccharomyces cerevisiae