Article
Physical and molecular genetic analysis of the multistep proteolytic maturation pathway utilized by vaccinia virus P4a protein.
The Journal of general virology - 1 Mar 1995
Whitehead S S, Bersani N A, Hruby D E
Abstract excerpt
Three potential AG*X cleavage sites have previously been identified in vaccinia virus (VV) P4a precursor, namely AG*N, AG*S and AG*T. Utilization of the COOH-proximal AG*T site in P4a leads to release of a 23 kDa product ('23K'). Here we propose that cleavage at the AG*S site alone is responsible for release of 4a, based on peptide mapping and microsequencing which demonstrated that the NH2 terminus of 4a is...
Topics
- Amino Acid Sequence
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Peptide Fragments
- Peptide Mapping
- Protein Precursors
- Protein Processing, Post-Translational
- Recombinant Fusion Proteins
- Sequence Analysis