Article
Functional analysis of mutations in the PIS gene, which encodes Saccharomyces cerevisiae phosphatidylinositol synthase.
FEMS microbiology letters - 1 Feb 1995
Kumano Y, Nikawa J
Abstract excerpt
The PIS gene for an enzyme phosphatidylinositol synthase having an increased Km for myo-inositol, was isolated from Saccharomyces cerevisiae. The mutant PIS gene contained a CAA codon at position 114 instead of the CAC codon observed in the wild-type gene, resulting in alteration of the amino acid from His to Gln. Oligonucleotide mediated site-directed mutagenesis of PIS at codon 114 revealed that mutant genes...
Topics
- CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase
- Codon
- Escherichia coli
- Genes, Fungal
- Histidine
- Inositol
- Mutagenesis, Site-Directed
- Mutation
- Recombinant Proteins
- Saccharomyces cerevisiae
- Sequence Analysis, DNA