Article
Cloning and sequence of the SCS3 gene which is required for inositol prototrophy in Saccharomyces cerevisiae.
Journal of biochemistry - 1 Dec 1994
Hosaka K, Nikawa J, Kodaki T, Ishizu H, Yamashita S
Abstract excerpt
The SCS3 gene of Saccharomyces cerevisiae was cloned by functional complementation, using a conditional mutant exhibiting myo-inositol auxotrophy in the presence of choline, and sequenced. The sequence contained an open reading frame capable of encoding 380 amino acids with a calculated molecular weight of 42,734. Disruption of the SCS3 locus caused myo-inositol auxotrophy. The gene appeared to be involved in the...
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