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Article

Streptomyces phospholipase D mutants with altered substrate specificity capable of phosphatidylinositol synthesis.

Chembiochem : a European journal of chemical biology - 14 Apr 2008

Masayama Atsushi, Takahashi Tetsuya, Tsukada Kaori, Nishikawa Seigo, Takahashi Rie, Adachi Masaatsu, Koga Kazushi, Suzuki Atsuo, Yamane Takashi, Nakano Hideo, Iwasaki Yugo

Abstract excerpt

The substrate specificity of a phospholipase D (PLD) from Streptomyces antibioticus was altered by site-directed saturation mutagenesis, so that it was able to synthesize phosphatidylinositol (PI). Mutations were introduced in the pld gene at the positions corresponding to three amino acid residues that might be involved in substrate recognition, and the mutated genes were expressed in Escherichia coli BL21...

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PMID: 18338352
DOI: 10.1002/cbic.200700528

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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