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Article

The catalytic role of aspartic acid-92 in a human dual-specific protein-tyrosine-phosphatase.

Biochemistry - 14 Mar 1995

Denu J M, Zhou G, Guo Y, Dixon J E

Abstract excerpt

The mechanism of catalysis for the human dual-specific (vaccinia H1-related) protein-tyrosine-phosphatase was investigated. The pH dependence of the kcat value is bell-shaped when p-nitrophenyl phosphate was employed as a model substrate. The kcat/Km pH profile rises with a slope of 2 and decreases with a slope of -1, indicating that two groups must be unprotonated and one group must be protonated for activity....

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Identifiers

PMID: 7880835
DOI: 10.1021/bi00010a031

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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