Article
Substrate positioning by His92 is important in catalysis by purple acid phosphatase.
The FEBS journal - 1 Jun 2005
Funhoff Enrico G, Wang Yunling, Andersson Goran, Averill Bruce A
Abstract excerpt
Proteolysis of single polypeptide mammalian purple acid phosphatases (PAPs) results in the loss of an interaction between the loop residue Asp146 and the active site residues Asn91 and/or His92. While Asn91 is a ligand to the divalent metal of the mixed-valent di-iron center, the role of His92 in the catalytic mechanism is unknown. Site-directed mutagenesis of His92 was performed to examine the role of this...
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