Spectroscopic and kinetic studies of the histidine residues of bovine low-molecular-weight phosphotyrosyl protein phosphatase.

doi:10.1021/bi00171a031

Back to search

Article

Spectroscopic and kinetic studies of the histidine residues of bovine low-molecular-weight phosphotyrosyl protein phosphatase.

Biochemistry - 8 Feb 1994

Davis J P, Zhou M M, Van Etten R L

Abstract excerpt

The role of the two conserved histidine residues in the low-molecular-weight phosphotyrosyl protein phosphatases was investigated using site-directed mutagenesis of the recombinant bovine heart enzyme. His-66 and His-72 were individually mutated to alanine and to asparagine. A double mutant, cont...

Topics

Animals
Base Sequence
Cattle
DNA Primers
Diethyl Pyrocarbonate
Histidine
Hydrolysis
Kinetics
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Weight
Mutation
Protein Tyrosine Phosphatases

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.