Article
Structural basis of potassium activation in plant asparaginases.
The FEBS journal - 1 Apr 2018
Ajewole Ebenezer, Santamaria-Kisiel Liliana, Pajak Agnieszka, Jaskolski Mariusz, Marsolais Frédéric
Abstract excerpt
l-asparaginases (EC 3.5.1.1) play an important role in nitrogen mobilization in plants. Here, we investigated the biochemical and biophysical properties of potassium-dependent (PvAspG1) and potassium-independent (PvAspG-T2) l-asparaginases from Phaseolus vulgaris. Our previous studies revealed that PvAspG1 requires potassium for catalytic activation and its crystal structure suggested that Ser-118 in the...
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