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Article

The roles of Tyr(91) and Lys(162) in general acid-base catalysis in the pigeon NADP+-dependent malic enzyme.

The Biochemical journal - 1 May 2008

Kuo Cheng-Chin, Lin Kuan-Yu, Hsu Yau-Jung, Lin Shu-Yu, Lin Yu-Tsen, Chang Gu-Gang, Chou Wei-Yuan

Abstract excerpt

The role of general acid-base catalysis in the enzymatic mechanism of NADP+-dependent malic enzyme was examined by detailed steady-state kinetic studies through site-directed mutagenesis of the Tyr(91) and Lys(162) residues in the putative catalytic site of the enzyme. Y91F and K162A mutants showed approx. 200- and 27000-fold decreases in k(cat) values respectively, which could be partially recovered with...

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PMID: 18248329
DOI: 10.1042/BJ20071278

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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