Basolateral secretion of amyloid precursor protein in Madin-Darby canine kidney cells is disturbed by alterations of intracellular pH and by introducing a mutation associated with familial Alzheimer's disease.

doi:10.1074/jbc.270.8.4058

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Article

Basolateral secretion of amyloid precursor protein in Madin-Darby canine kidney cells is disturbed by alterations of intracellular pH and by introducing a mutation associated with familial Alzheimer's disease.

The Journal of biological chemistry - 24 Feb 1995

De Strooper B, Craessaerts K, Dewachter I, Moechars D, Greenberg B, Van Leuven F, Van den Berghe H

Abstract excerpt

The analysis of potential sorting signals in amyloid precursor protein (APP) by site-directed mutagenesis and the disturbance of metabolic pathways by drugs is used here to define the parameters that determine polarized secretion of APP in Madin-Darby canine kidney cells. Endogenously produced APP751/770 and APP695 produced from transfected constructs are secreted almost exclusively into the basolateral...

Topics

Alzheimer Disease
Amyloid Precursor Protein Secretases
Amyloid beta-Protein Precursor
Animals
Aspartic Acid Endopeptidases
Cell Compartmentation
Cell Line
Cell Membrane
Cytoplasm

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Dogs

Endopeptidases

Hydrogen-Ion Concentration

Kidney

Mice

Mutation

Transfection

Source

Open in PubMed

Identifiers

PMID: 7876155
DOI: 10.1074/jbc.270.8.4058

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7876155-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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