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Article

Transmembrane segment II of NhaA Na+/H+ antiporter lines the cation passage, and Asp65 is critical for pH activation of the antiporter.

The Journal of biological chemistry - 15 Jan 2010

Herz Katia, Rimon Abraham, Olkhova Elena, Kozachkov Lena, Padan Etana

Abstract excerpt

The crystal structure of Escherichia coli NhaA determined at pH 4 has provided insights into the mechanism of activity of a pH-regulated Na+/H+ antiporter. However, because NhaA is activated at physiological pH (pH 5.5-8.5), many questions related to the active state of NhaA have remained elusive. Our experimental results at physiological pH and computational analyses reveal that amino acid residues in...

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Identifiers

PMID: 19923224
DOI: 10.1074/jbc.M109.047134
PMCID: PMC2804377

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