Article
Fluorescence resolution of the intrinsic tryptophan residues of bovine protein tyrosyl phosphatase.
The Journal of biological chemistry - 24 Feb 1995
Pokalsky C, Wick P, Harms E, Lytle F E, Van Etten R L
Abstract excerpt
Fluorescence steady-state and lifetime measurements have been performed that permit the differentiation of the 2 intrinsic tryptophan residues in bovine low molecular weight phosphotyrosyl protein phosphatase (BPTP). Spectral information was obtained by use of two single-tryptophan mutant protein...
Topics
- Animals
- Binding Sites
- Catalysis
- Cattle
- Hydrogen-Ion Concentration
- Mutation
- Protein Folding
- Protein Tyrosine Phosphatases
- Spectrometry, Fluorescence
- Tryptophan