Article
Isolation of a human cDNA that complements a mutant hamster cell defective in methotrexate uptake.
The Journal of biological chemistry - 17 Feb 1995
Williams F M, Flintoff W F
Abstract excerpt
A clone has been isolated from a human lymphoblastic cDNA expression library that complements a mutant Chinese hamster cell defective in the uptake of the folate analogue methotrexate. When transfected with this clone the mutant cells regain the ability to transport the drug and, as a consequence...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Biological Transport
- CHO Cells
- Carcinoma, Hepatocellular
- Carrier Proteins
- Cricetinae
- DNA, Complementary
- Female
- Folate Receptors, GPI-Anchored
- Genetic Complementation Test
- Humans
- Kinetics
- Liver Neoplasms
- Methotrexate
- Mice
- Molecular Sequence Data