Article
Isolation of a hamster cDNA clone coding for a function involved in methotrexate uptake.
The Journal of biological chemistry - 25 Feb 1994
Williams F M, Murray R C, Underhill T M, Flintoff W F
Abstract excerpt
A clone has been isolated from a Chinese hamster ovary cell cDNA expression library that complements mutant cells defective in the uptake of the folate analogue methotrexate. When transfected with this clone, the mutant cells regain the ability to bind and transport the drug and, as a consequence, become sensitive to its cytotoxic action. The clone is 2314 base pairs long and has an open reading frame of 1557...
Topics
- ATP Binding Cassette Transporter, Subfamily G, Member 2
- ATP-Binding Cassette Transporters
- Amino Acid Sequence
- Animals
- Base Sequence
- Biological Transport
- Blotting, Northern
- CHO Cells
- Carrier Proteins
- Cricetinae