Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Isolation of a hamster cDNA clone coding for a function involved in methotrexate uptake.

The Journal of biological chemistry - 25 Feb 1994

Williams F M, Murray R C, Underhill T M, Flintoff W F

Abstract excerpt

A clone has been isolated from a Chinese hamster ovary cell cDNA expression library that complements mutant cells defective in the uptake of the folate analogue methotrexate. When transfected with this clone, the mutant cells regain the ability to bind and transport the drug and, as a consequence, become sensitive to its cytotoxic action. The clone is 2314 base pairs long and has an open reading frame of 1557...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to Base Sequence
Share to Carrier Proteins

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Cricetinae

Share to DNA, Complementary

Share to Molecular Sequence Data

Share to Mutation

Share to Phenotype

Source

Identifiers

PMID: 8119923

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Isolation of a hamster cDNA clone coding for a function involved in methotrexate uptake.PMID 8119923Read ↗

Isolation of a human cDNA that complements a mutant hamster cell defective in methotrexate uptake.Semantic proximity Read ↗

Isolation of Human cDNAs That Restore Methotrexate Sensitivity and Reduced Folate Carrier Activity in Methotrexate Transport-defective Chinese Hamster Ovary CellsSemantic proximity Read ↗

Reduced folate carrier: biochemistry and molecular biology of the normal and methotrexate-resistant cell.Semantic proximity Read ↗

Characterization of a mutation in the reduced folate carrier in a transport defective L1210 murine leukemia cell line.Semantic proximity Read ↗

Methotrexate Transport and ResistanceSemantic proximity Read ↗

Functional analysis of altered reduced folate carrier sequence changes identified in osteosarcomas.Semantic proximity Read ↗

Mutations in the reduced-folate carrier affect protein localization and stability.Semantic proximity Read ↗

Impaired membrane transport in methotrexate-resistant CCRF-CEM cells involves early translation termination and increased turnover of a mutant reduced folate carrier.Semantic proximity Read ↗

A reduced folate carrier mutation produces substrate-dependent alterations in carrier mobility in murine leukemia cells and methotrexate resistance with conservation of growth in 5-formyltetrahydrofolate.Semantic proximity Read ↗

Retrovirally mediated complementation of the glyB phenotype. Cloning of a human gene encoding the carrier for entry of folates into mitochondria.Semantic proximity Read ↗

Methylation-dependent Silencing of the Reduced Folate Carrier Gene in Inherently Methotrexate-resistant Human Breast Cancer CellsSemantic proximity Read ↗

Cloning of the Human Thiamine Transporter, a Member of the Folate Transporter FamilySemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.