Article
Site-specific conjugation of an enzyme and an antibody fragment.
Bioconjugate chemistry - 1 Jan 2000
Werlen R C, Lankinen M, Rose K, Blakey D, Shuttleworth H, Melton R, Offord R E
Abstract excerpt
A site-specific immunoconjugate was prepared between an F(ab')2-like fragment of the monoclonal anti-CEA murine IgG1 A5B7 and a mutant of the dimeric enzyme carboxypeptidase G2 possessing an N-terminal Thr in place of Ala. First an aldehyde was introduced at the N-terminus of the enzyme by mild p...
Topics
- Antibodies, Monoclonal
- Antibodies, Neoplasm
- Antigen-Antibody Reactions
- Carcinoembryonic Antigen
- Chromatography, Ion Exchange
- Electrophoresis, Polyacrylamide Gel
- Hydrazones
- Hydrolysis
- Immunoconjugates
- Immunoglobulin Fab Fragments
- Mass Spectrometry
- Mutation
- Oxidation-Reduction
- Prodrugs
- gamma-Glutamyl Hydrolase