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Article

Evidence for substrate stabilization in regulation of the degradation of Bacillus subtilis aspartate transcarbamylase in vivo.

Archives of biochemistry and biophysics - 10 Jan 1995

Hu P, Switzer R L

Abstract excerpt

Aspartate transcarbamylase (ATCase) is rapidly degraded in Bacillus subtilis cells that are starved for a carbon or nitrogen source or a required amino acid. The hypothesis that ATCase degradation may be regulated in vivo by protection of the enzyme by substrate binding was tested by studies of a mutant ATCase (Arg99 to Ala, R99A), which binds substrate so poorly that it fails to support pyrimidine-independent...

Topics

Aspartate Carbamoyltransferase
Aspartic Acid
Bacillus subtilis
Carbamyl Phosphate
Circular Dichroism
Enzyme Stability
Guanidine
Guanidines
Hot Temperature
Ligases
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Denaturation

Recombinant Proteins

Subtilisins

Source

Identifiers

PMID: 7840626
DOI: 10.1006/abbi.1995.1036

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7840626-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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