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Article

Importance of residues Arg-167 and Gln-231 in both the allosteric and catalytic mechanisms of Escherichia coli aspartate transcarbamoylase.

Biochemistry - 24 Apr 1990

Stebbins J W, Zhang Y, Kantrowitz E R

Abstract excerpt

Site-specific mutagenesis has been used to create two mutant versions of aspartate transcarbamoylase. Arg-167 and Gln-231, both previously identified as interacting with the portion of the bisubstrate analogue N-(phosphonoacetyl)-L-aspartate (PALA) that corresponds to aspartate [Krause, K. L., Voltz, K. W., & Lipscomb, W. N. (1987) J. Mol. Biol. 193, 527-553], were replaced by glutamine and leucine, respectively....

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Identifiers

PMID: 2191720
DOI: 10.1021/bi00468a003

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