Article
Human ubiquitin-activating enzyme, E1. Indication of potential nuclear and cytoplasmic subpopulations using epitope-tagged cDNA constructs.
The Journal of biological chemistry - 30 Dec 1994
Handley-Gearhart P M, Stephen A G, Trausch-Azar J S, Ciechanover A, Schwartz A L
Abstract excerpt
The ubiquitin-activating enzyme E1 catalyzes the first step in the ubiquitin conjugation pathway. Previously, we have cloned and sequenced the cDNA for human E1. Expression of the E1 cDNA in the ts20 cell line, which harbors a thermolabile E1, abrogated the phenotypic defects associated with this...
Topics
- Amino Acid Sequence
- Cell Nucleus
- Cytoplasm
- DNA, Complementary
- Epitopes
- Fluorescent Antibody Technique
- HeLa Cells
- Humans
- Ligases
- Molecular Sequence Data
- Mutation
- Phenotype
- Transfection
- Ubiquitin-Activating Enzymes
- Ubiquitin-Protein Ligases