Article
A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution.
The EMBO journal - 15 Dec 1994
Doye V, Wepf R, Hurt E C
Abstract excerpt
Temperature-sensitive nucleoporin nup49-316 mutant cells accumulate poly(A)+ RNA inside the nucleus when shifted to restrictive temperature. We performed a synthetic lethal screen with this mutant allele to identify further components of the mRNA export machinery. A synthetic lethal mutant slv21...
Topics
- Amino Acid Sequence
- Biological Transport
- Cell Compartmentation
- Cloning, Molecular
- Fluorescent Antibody Technique
- Genes, Fungal
- Membrane Proteins
- Microscopy, Electron
- Molecular Sequence Data
- Mutation
- Nuclear Envelope
- Nuclear Pore Complex Proteins
- Nuclear Proteins
- RNA, Messenger
- Saccharomyces cerevisiae Proteins
- Selection, Genetic
- Sequence Analysis, DNA
- Yeasts