Article
Mutation or deletion of the Saccharomyces cerevisiae RAT3/NUP133 gene causes temperature-dependent nuclear accumulation of poly(A)+ RNA and constitutive clustering of nuclear pore complexes.
Molecular biology of the cell - 1 Apr 1995
Li O, Heath C V, Amberg D C, Dockendorff T C, Copeland C S, Snyder M, Cole C N
Abstract excerpt
To identify genes whose products play potential roles in the nucleocytoplasmic export of messenger RNA, we isolated temperature-sensitive strains of Saccharomyces cerevisiae and examined them by fluorescent in situ hybridization. With the use of a digoxigen-tagged oligo-(dT)50 probe, we identifie...
Topics
- Amino Acid Sequence
- Base Sequence
- Biological Transport
- Cell Nucleus
- Chromosome Mapping
- Fungal Proteins
- Gene Deletion
- Gene Expression Regulation, Fungal
- Genes, Fungal
- In Situ Hybridization, Fluorescence