PCR restriction analysis of genome composition and stability of cold-adapted reassortant live influenza vaccines.

doi:10.1016/0166-0934(94)00133-2

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Article

PCR restriction analysis of genome composition and stability of cold-adapted reassortant live influenza vaccines.

Journal of virological methods - 1 Mar 1995

Klimov A I, Cox N J

Abstract excerpt

Using cold-adapted master donor strains of influenza virus as a model, an approach was developed that exploits unique nucleotide differences between the donor strains and wild-type influenza viruses for rapidly and simply determining the genome composition and genetic stability of live attenuated...

Topics

Acclimatization
Adult
Allantois
Base Sequence
Child
Cold Temperature
DNA Primers
Genome, Viral
Humans
Influenza A virus
Influenza Vaccines
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Restriction Mapping
Virion

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