Article
Sequence of the Kluyveromyces lactis beta-galactosidase: comparison with prokaryotic enzymes and secondary structure analysis.
Gene - 1 Sept 1992
Poch O, L'Hôte H, Dallery V, Debeaux F, Fleer R, Sodoyer R
Abstract excerpt
The LAC4 gene encoding the beta-galactosidase (beta Gal) of the yeast, Kluyveromyces lactis, was cloned on a 7.2-kb fragment by complementation of a lacZ-deficient Escherichia coli strain. The nucleotide sequence of the structural gene, with 42 bp and 583 bp of the 5'- and 3'-flanking sequences, respectively, was determined. The deduced amino acid (aa) sequence of the K. lactis beta Gal predicts a 1025-aa...
Topics
- Amino Acid Sequence
- Base Sequence
- Biological Evolution
- Cloning, Molecular
- Eukaryotic Cells
- Genes, Fungal
- Genetic Variation
- Glucuronidase
- Kluyveromyces
- Molecular Sequence Data
- Prokaryotic Cells