Article
Cloning and analysis of the Kluyveromyces lactis TRP1 gene: a chromosomal locus flanked by genes encoding inorganic pyrophosphatase and histone H3.
Yeast (Chichester, England) - 1 Jan 2000
Stark M J, Milner J S
Abstract excerpt
The TRP1 gene of the yeast Kluyveromyces lactis has been cloned from a genomic library by complementation of the Saccharomyces cerevisiae trp1-289 mutation. The gene was located within the clone by transposon mutagenesis and the coding region identified by DNA sequencing. This has indicated that...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Fungal
- Genes, Fungal
- Histones
- Kluyveromyces
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Plasmids
- Pyrophosphatases
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomycetales