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Article

Cloning of the nitrate-nitrite reductase gene cluster of Penicillium chrysogenum and use of the niaD gene as a homologous selection marker.

Journal of biotechnology - 1 Sept 1991

Gouka R J, van Hartingsveldt W, Bovenberg R A, van den Hondel C A, van Gorcom R F

Abstract excerpt

A new homologous transformation system for the filamentous fungus Penicillium chrysogenum is described. The system is based on complementation of niaD mutants using the nitrate reductase structural gene (niaD) of P. chrysogenum. Spontaneous niaD mutants were identified after selection for chlorate resistance, in growth tests and subsequent complementation with the niaD gene of Aspergillus oryzae. The P....

Topics

Cloning, Molecular
DNA, Fungal
Genes, Fungal
Genetic Markers
Multigene Family
Mutation
Nitrate Reductase
Nitrate Reductases
Nitrite Reductases
Penicillium chrysogenum

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1367546
DOI: 10.1016/0168-1656(91)90227-m

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1367546-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Cloning of the nitrate-nitrite reductase gene cluster of Penicillium chrysogenum and use of the niaD gene as a homologous selection marker.PMID 1367546Read ↗

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