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Article

Engineering Yarrowia lipolytica to express secretory invertase with strong FBA1IN promoter.

Yeast (Chichester, England) - 1 Feb 2012

Hong Seung-Pyo, Seip John, Walters-Pollak Dana, Rupert Ross, Jackson Raymond, Xue Zhixiong, Zhu Quinn

Abstract excerpt

Oleaginous yeast Yarrowia lipolytica is an important host for the production of lipid-derived compounds or heterologous proteins. Selection of strong promoters and effective expression systems is critical for heterologous protein secretion. To search for a strong promoter in Y. lipolytica, activities of FBA1, TDH1 and GPM1 promoters were compared to that of TEF1 promoter by constructing GUS reporter fusions. The...

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PMID: 22222800
DOI: 10.1002/yea.1917

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