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Article

Engineering Yarrowia lipolytica to express secretory invertase with strong FBA1IN promoter.

Yeast (Chichester, England) - 1 Feb 2012

Hong Seung-Pyo, Seip John, Walters-Pollak Dana, Rupert Ross, Jackson Raymond, Xue Zhixiong, Zhu Quinn

Abstract excerpt

Oleaginous yeast Yarrowia lipolytica is an important host for the production of lipid-derived compounds or heterologous proteins. Selection of strong promoters and effective expression systems is critical for heterologous protein secretion. To search for a strong promoter in Y. lipolytica, activities of FBA1, TDH1 and GPM1 promoters were compared to that of TEF1 promoter by constructing GUS reporter fusions. The...

Topics

Base Sequence
Fungal Proteins
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Genes, Reporter
Genetic Engineering
Genetic Vectors
Molecular Sequence Data
Phenotype
Promoter Regions, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sucrose

Yarrowia

beta-Fructofuranosidase

Source

Identifiers

PMID: 22222800
DOI: 10.1002/yea.1917

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