Efficient production of a functional single-chain antidigoxin antibody via an engineered Bacillus subtilis expression-secretion system.

doi:10.1038/nbt0193-71

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Article

Efficient production of a functional single-chain antidigoxin antibody via an engineered Bacillus subtilis expression-secretion system.

Bio/technology (Nature Publishing Company) - 1 Jan 1993

Wu X C, Ng S C, Near R I, Wong S L

Abstract excerpt

We have applied a Bacillus subtilis expression-secretion system to produce a functional antidigoxin SCA (single-chain antibody consisting of VL-linker-VH) and the individual variable domains of light (VL) and heavy (VH) chains. The secreted antidigoxin SCA can be affinity purified in one step by applying the culture supernatant directly to a ouabain-Sepharose column. N-terminal sequence determination indicated...

Topics

Amino Acid Sequence
Antibodies
Antibody Specificity
Bacillus subtilis
Base Sequence
Chromatography, Affinity
Digoxin
Genes
Genes, Immunoglobulin
Genetic Engineering
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Immunoglobulin Heavy Chains

Immunoglobulin Light Chains

Immunoglobulin Variable Region

Molecular Sequence Data

Oligodeoxyribonucleotides

Phenotype

Plasmids

Source

Open in PubMed

Identifiers

PMID: 7763487
DOI: 10.1038/nbt0193-71

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7763487-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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