Article
Enhanced secretory production of a single-chain antibody fragment from Bacillus subtilis by coproduction of molecular chaperones.
Journal of bacteriology - 1 Jun 1998
Wu S C, Ye R, Wu X C, Ng S C, Wong S L
Abstract excerpt
Formation of inclusion bodies is a major limiting factor for secretory production of an antidigoxin single-chain antibody (SCA) fragment from Bacillus subtilis. To address this problem, three new strains with enhanced production of molecular chaperones were constructed. WB600BHM constitutively pr...
Topics
- Antigen-Antibody Reactions
- Bacillus subtilis
- Bacterial Proteins
- Cell Wall
- Chaperonin 10
- Chaperonin 60
- Chaperonins
- DNA-Binding Proteins
- Digoxin
- Escherichia coli Proteins
- Genetic Vectors
- HSP70 Heat-Shock Proteins
- Heat-Shock Proteins
- Immunoglobulin Fragments
- Kinetics
- Lipoproteins
- Membrane Proteins
- Molecular Chaperones