Article
Broad specificity immunoassay for detection of Bacillus thuringiensis Cry toxins through engineering of a single chain variable fragment with mutagenesis and screening.
International journal of biological macromolecules - 1 Feb 2018
Zhong Jianfeng, Hu Xiaodan, Zhang Xiao, Liu Yuan, Xu Chongxin, Zhang Cunzheng, Lin Manman, Liu Xianjin
Abstract excerpt
The cultivation of genetically modified crops (GMCs) has greatly increased worldwide. Given the fact that over 10 Bacillus thuringiensis Cry toxins have been applied in GMCs, there is a need to develop an efficient and economically affordable detection method that simultaneously screen such compounds with similar structures in crops and foodstuff. Here we described an approach using a site-directed mutagenesis...
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