Article
Different PrlA proteins increase the efficiency of periplasmic production of human interleukin-6 in Escherichia coli.
Journal of biotechnology - 20 Aug 1996
Pérez-Pérez J, Barbero J L, Márquez G, Gutiérrez J
Abstract excerpt
The export efficiency of a fusion of the Escherichia coli preOmpA signal peptide to human interleukin-6 can be significantly raised by coexpressing three different prlA alleles of sec Y along with wild type secE. The effect seems prlA-specific, as prlG1 (a prl allele of secE) does not affect the...
Topics
- Alleles
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Biological Transport
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Humans
- Interleukin-6
- Membrane Proteins
- Membrane Transport Proteins
- Mutation
- Recombinant Proteins
- SEC Translocation Channels