Cell-substrate interactions and locomotion of Dictyostelium wild-type and mutants defective in three cytoskeletal proteins: a study using quantitative reflection interference contrast microscopy.

Abstract excerpt

Reflection interference contrast microscopy combined with digital image processing was applied to study the motion of Dictyostelium discoideum cells in their pre-aggregative state on substrata of different adhesiveness (glass, albumin-covered glass, and freshly cleaved mica). The temporal variations of the size and shape of the cell/substratum contact area and the time course of advancement of pseudopods...

Topics

• Animals
• Biophysical Phenomena
• Biophysics
• Cell Adhesion
• Cell Movement
• Cytoskeletal Proteins
• Dictyostelium
• Image Processing, Computer-Assisted
• Microscopy, Interference
• Mutation