Article
Reversibility of DnaA protein activity in the 'irreversible' dnaA204 mutant of Escherichia coli.
Molecular microbiology - 1 Jan 1995
Hansen F G, Atlung T
Abstract excerpt
The dnaA204 mutant, one of the so-called irreversible dnaA mutants which cannot reinitiate chromosome replication upon a shift from non-permissive to permissive growth temperature in the absence of protein synthesis, was reinvestigated using flow cytometry and marker frequency analysis. In a temperature down-shift experiment and in the presence of protein synthesis the dnaA204 mutant reinitiates chromosome...
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