Article
Reversibility of DnaA protein activity in the 'irreversible' dnaA204 mutant of Escherichia coli.
Molecular microbiology - 1 Jan 1995
Hansen F G, Atlung T
Abstract excerpt
The dnaA204 mutant, one of the so-called irreversible dnaA mutants which cannot reinitiate chromosome replication upon a shift from non-permissive to permissive growth temperature in the absence of protein synthesis, was reinvestigated using flow cytometry and marker frequency analysis. In a temperature down-shift experiment and in the presence of protein synthesis the dnaA204 mutant reinitiates chromosome...
Topics
- Bacterial Proteins
- DNA Replication
- DNA-Binding Proteins
- Escherichia coli
- Gene Expression
- Kinetics
- Mutation
- Protein Denaturation
- Protein Folding
- Replication Origin
- Temperature