Abnormal integrity of the nucleolus associated with cell cycle arrest owing to the temperature-sensitive ubiquitin-activating enzyme E1.

doi:10.1007/BF00710672

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Article

Abnormal integrity of the nucleolus associated with cell cycle arrest owing to the temperature-sensitive ubiquitin-activating enzyme E1.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology - 1 Mar 1995

Sudha T, Tsuji H, Sameshima M, Matsuda Y, Kaneda S, Nagai Y, Yamao F, Seno T

Abstract excerpt

A mouse cell mutant, ts85, containing the temperature-sensitive ubiquitin-activating enzyme was arrested in G2 phase at the non-permissive temperature. In the arrested cells, azure C, a nucleolus-specific stain, revealed a U-shaped or ring-shaped arrangement of nucleolar lobes with an unstained region in the center. Silver staining of the nucleolar organizer region (NOR) and fluorescence in situ hybridization...

Topics

Animals
Cell Cycle
Cell Division
Cell Nucleolus
Chromosome Aberrations
DNA, Ribosomal
In Situ Hybridization, Fluorescence
Interphase
Ligases
Mice

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Microscopy, Electron

Mutation

Nucleolus Organizer Region

Silver Staining

Tumor Cells, Cultured

Ubiquitin-Activating Enzymes

Ubiquitin-Protein Ligases

Ubiquitins

Source

Open in PubMed

Identifiers

PMID: 7749560
DOI: 10.1007/BF00710672

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7749560-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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