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Article

Regulated expression of the Alcaligenes eutrophus pha biosynthesis genes in Escherichia coli.

Applied and environmental microbiology - 1 Apr 1995

Kidwell J, Valentin H E, Dennis D

Abstract excerpt

A novel poly-beta-hydroxybutyrate (PHB) production system in which the expression and gene dosage of the Alcaligenes eutrophus pha biosynthetic operon were effectively regulated by cultivation temperature was constructed in Escherichia coli. The pha operon was fused to the negatively regulated tac promoter and cloned into a vector in which the copy number is temperature dependent. A two-phase process was employed...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 7747959
DOI: 10.1128/aem.61.4.1391-1398.1995
PMCID: PMC167396

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Source receipt: pubmed, retrieved 7 Sept 2026.

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