Regulated expression of the Alcaligenes eutrophus pha biosynthesis genes in Escherichia coli.

doi:10.1128/aem.61.4.1391-1398.1995

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Article

Regulated expression of the Alcaligenes eutrophus pha biosynthesis genes in Escherichia coli.

Applied and environmental microbiology - 1 Apr 1995

Kidwell J, Valentin H E, Dennis D

Abstract excerpt

A novel poly-beta-hydroxybutyrate (PHB) production system in which the expression and gene dosage of the Alcaligenes eutrophus pha biosynthetic operon were effectively regulated by cultivation temperature was constructed in Escherichia coli. The pha operon was fused to the negatively regulated ta...

Topics

Alcaligenes
Cloning, Molecular
Drug Resistance, Microbial
Escherichia coli
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genetic Vectors
Hydroxybutyrates
Operon
Phenotype
Polyesters
Promoter Regions, Genetic

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