Article
Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442.
Bioresource technology - 1 Apr 2009
Cai Lei, Yuan Mei-Qing, Liu Feng, Jian Jia, Chen Guo-Qiang
Abstract excerpt
Pseudomonas putida KT2442 is a medium-chain-length polyhydroxyalkanoates (PHA) producer. One of the main shortages in the production of PHA has been the intracellular PHA degradation caused by its endogenous PHA depolymerase. The aim of this study was to improve PHA production via removing the PHA degradation mechanism. PHA depolymerase phaZ knockout mutant P. putida KTMQ01 was successfully constructed, which...
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