Article
A cold-passaged, attenuated strain of human respiratory syncytial virus contains mutations in the F and L genes.
Virology - 20 Apr 1995
Connors M, Crowe J E, Firestone C Y, Murphy B R, Collins P L
Abstract excerpt
In previous studies, a mutant (cp-RSV) of the RSV A2 strain derived from 52 serial cold passages in bovine embryonic tissue culture was highly attenuated in seropositive adults and children but caused upper respiratory tract disease in seronegative infants. We investigated the genetic basis for t...
Topics
- Amino Acids
- Cloning, Molecular
- Cold Temperature
- Genes, Viral
- Genetic Variation
- Genome, Viral
- HN Protein
- Humans
- Mutation
- Open Reading Frames
- Phenotype
- RNA, Viral
- Respiratory Syncytial Viruses
- Sequence Analysis, DNA
- Serial Passage
- Vaccines, Attenuated
- Viral Envelope Proteins
- Viral Proteins