Article
A yeast protein that bidirectionally affects nucleocytoplasmic transport.
Journal of cell science - 1 Jan 1995
Singleton D R, Chen S, Hitomi M, Kumagai C, Tartakoff A M
Abstract excerpt
We have identified a temperature-sensitive mutant of Saccharomyces cerevisiae (npl3) that accumulates polyadenylated RNA in the nucleus at 37 degrees C, as judged by in situ hybridization. The strong nuclear signal is not simply due to increased cytoplasmic turnover of mRNA, as reincubation at 37...
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Nucleus
- Cloning, Molecular
- Cytoplasm
- DNA Primers
- Fungal Proteins
- Genes, Fungal
- Genetic Complementation Test
- Histones
- In Situ Hybridization
- Kinetics
- Methionine
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- Polymerase Chain Reaction
- RNA, Fungal
