Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Validation of allele-specific polymerase chain reaction for DNA typing of HLA-B27.

Clinical chemistry - 1 May 1995

Steffens-Nakken H M, Zwart G, van den Bergh F A

Abstract excerpt

To find a specific method for HLA-B27 typing for the diagnosis of rheumatic disorders, we extensively tested the single-step B27-specific polymerase chain reaction (PCR) described by Dominguez et al. (Immunogenetics 1992;36:277-82). This method, which relies on specific primer recognition of a sequence in the third exon (unique to the B27-allele), was used for screening of 270 characterized blood samples, 57 of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Base Sequence
Share to DNA

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Flow Cytometry

Share to Molecular Sequence Data

Share to Nucleic Acid Hybridization

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 7729046

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Validation of allele-specific polymerase chain reaction for DNA typing of HLA-B27.PMID 7729046Read ↗

Rapid HLA-B27 screening with real-time TaqMan PCR: a clinical validation in the Dutch population.Semantic proximity Read ↗

Rapid detection of all HLA-B*27 alleles (B*2701-B*2725) by group-specific polymerase chain reaction.Semantic proximity Read ↗

A high-resolution polymerase chain reaction-sequence-specific primer HLA-B*27 typing set and its application in routine HLA-B27 testing.Semantic proximity Read ↗

HLA-B*27 typing by PCR-restriction fragment length polymorphism.Semantic proximity Read ↗

A Cost-Effective and Labor-Saving Method for Detecting Human Leukocyte Antigen B27 Status via Sequence-Encoded Fluorescence Amplification Assay.Semantic proximity Read ↗

A multicenter international evaluation of single-tube amplification protocols for sequencing-based typing of HLA-DRB1 and HLA-DRB3,4,5.Semantic proximity Read ↗

Rapid DNA typing of HLA-B27 allele by real-time PCR using lightcycler technology.Semantic proximity Read ↗

HLA-B27 genotyping by fluorescent resonance emission transfer (FRET) probes in real-time PCR.Semantic proximity Read ↗

HLA-B27 typing: evaluation of an allele-specific PCR melting assay and two flow cytometric antigen assays.Semantic proximity Read ↗

HLA-B27 determination by polymerase chain reaction.Semantic proximity Read ↗

An integrated multiplex-PCR and PCR-RFLP typing system for markers associated with seronegative arthritides.Semantic proximity Read ↗

Two color analysis of HLA-B27 antigen by flow cytometer--a comparative study by conventional microlymphocytotoxicity, DNA genotyping polymerase chain reaction and flow cytometric measurement.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.