Validation of allele-specific polymerase chain reaction for DNA typing of HLA-B27.

Article

Validation of allele-specific polymerase chain reaction for DNA typing of HLA-B27.

Clinical chemistry - 1 May 1995

Steffens-Nakken H M, Zwart G, van den Bergh F A

Abstract excerpt

To find a specific method for HLA-B27 typing for the diagnosis of rheumatic disorders, we extensively tested the single-step B27-specific polymerase chain reaction (PCR) described by Dominguez et al. (Immunogenetics 1992;36:277-82). This method, which relies on specific primer recognition of a se...

Topics

Alleles
Base Sequence
DNA
Exons
Flow Cytometry
Globins
HLA-B27 Antigen
Humans
Molecular Sequence Data
Nucleic Acid Hybridization
Polymerase Chain Reaction
Rheumatic Diseases

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