Article
Structural requirements for addition of O-linked carbohydrate to recombinant erythropoietin.
Biochemistry - 20 Sept 1994
Elliott S, Bartley T, Delorme E, Derby P, Hunt R, Lorenzini T, Parker V, Rohde M F, Stoney K
Abstract excerpt
To define the structural requirements for addition of O-linked glycosylation in vivo, recombinant erythropoietin (rEPO) variants were constructed. Thirty-three independent Ser or Thr substitutions were constructed and examined to see which were subject to O-linked carbohydrate addition. Variants...
Topics
- Amino Acid Sequence
- Animals
- Blotting, Western
- CHO Cells
- Cell Line
- Chlorocebus aethiops
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- Cricetinae
- Disulfides
- Erythropoietin
- Genetic Variation
- Glycosylation
- Humans
- Isoelectric Focusing
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Neuraminidase