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Article

Mapping of the Active Site of Recombinant Human Erythropoietin

15 Jan 1997

Abstract excerpt

Recombinant human erythropoietin (rHuEPO) variants have been constructed to identify amino acid residues important for biological activity. Immunoassays were used to determine the effect of each mutation on rHuEPO folding. With this strategy, we could distinguish between mutations that affected bioactivity directly and those that affected bioactivity because the mutation altered rHuEPO conformation. Four regions...

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PMID: 9002951
DOI: 10.1182/blood.v89.2.493

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