Article
Seven new mutations in hMSH2, an HNPCC gene, identified by denaturing gradient-gel electrophoresis.
American journal of human genetics - 1 May 1995
Wijnen J, Vasen H, Khan P M, Menko F H, van der Klift H, van Leeuwen C, van den Broek M, van Leeuwen-Cornelisse I, Nagengast F, Meijers-Heijboer A
Abstract excerpt
Hereditary nonpolyposis colorectal cancer (HNPCC) is a relatively common autosomal dominant cancer-susceptibility condition. The recent isolation of the DNA mismatch repair genes (hMSH2, hMLH1, hPMS1, and hPMS2) responsible for HNPCC has allowed the search for germ-line mutations in affected individuals. In this study we used denaturing gradient-gel electrophoresis to screen for mutations in the hMSH2 gene....
Topics
- Base Sequence
- Colorectal Neoplasms, Hereditary Nonpolyposis
- DNA-Binding Proteins
- Denmark
- Electrophoresis
- Exons
- Humans
- Italy
- Molecular Sequence Data
- MutS Homolog 2 Protein