Article
Mapping of the pulmonary surfactant SP5 (SFTP2) locus to 8p21 and characterization of a microsatellite repeat marker that shows frequent loss of heterozygosity in human carcinomas.
Genomics - 1 Dec 1994
Wood S, Yaremko M L, Schertzer M, Kelemen P R, Minna J, Westbrook C A
Abstract excerpt
We have identified 4 cosmids at the SFTP2 locus by cDNA hybridization. SFTP2 was mapped using a polymorphic CA repeat and localized to 8p21 by FISH. Allele loss in carcinomas was detected using this PCR marker. Among 11 lung and colon tumors, 6 of 9 informative cases exhibited allelic loss.
Topics
- Alleles
- Base Sequence
- Chromosome Mapping
- Chromosomes, Human, Pair 8
- Cosmids
- DNA, Satellite
- Genetic Linkage
- Genetic Markers
- Heterozygote
- Humans
- In Situ Hybridization, Fluorescence
- Molecular Sequence Data
- Neoplasms
- Polymerase Chain Reaction
- Pulmonary Surfactants