Article
A "trans-acting" factor for activation of transcription is defective in the xrs-5 mutant of the Chinese hamster ovary cell line.
Mutation research - 1 Aug 1993
Zhu W, Kriajevskaia M, Keng P C, Chou W G
Abstract excerpt
A cDNA molecule (B52) with the characteristics of retroviral sequences was isolated from the Chinese hamster ovary (CHO) K1 cell line. B52 cDNA detected two species of mRNAs with the size of 5.2 and 2.7 Kb in CHO cells. However, the steady-state level of the two mRNAs was reduced 26-fold in the X-ray-sensitive mutants, xrs-5 and xrs-6. Several experiments were performed to study the mechanism of underexpression....
Topics
- Animals
- CHO Cells
- Cricetinae
- Gene Expression Regulation
- Mutation
- Promoter Regions, Genetic
- RNA, Messenger
- Repetitive Sequences, Nucleic Acid
- Trans-Activators
- Transcriptional Activation