Article
Molecular cloning and characterization of the RNA packaging-defective retrovirus SE21Q1b.
Journal of virology - 1 Jan 1992
Anderson D J, Lee P, Levine K L, Sang J S, Shah S A, Yang O O, Shank P R, Linial M L
Abstract excerpt
The nonconditional RNA packaging mutant SE21Q1b contains cis- and trans-acting defects which cause cellular mRNA, rather than viral genomic RNA, to be nonspecifically packaged into SE21Q1b viral particles. Using genomic libraries of the c-SE21Q1b quail cell line, we have been able to construct a molecular clone of the SE21Q1b provirus. Upon transfection into primary quail embryo fibroblasts, the SE21Q1b molecular...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cell Line
- Cloning, Molecular
- DNA, Viral
- Defective Viruses
- Exons
- Gene Products, gag
- Molecular Sequence Data