Article
Detection of p53 mutations in benign and dysplastic nevi.
Cancer research - 1 Oct 1995
Levin D B, Wilson K, Valadares de Amorim G, Webber J, Kenny P, Kusser W
Abstract excerpt
We have examined melanocytic cells derived directly from fresh biopsy tissue for the presence of p53 mutations. Using selective media that permits growth of melanocytes and inhibits growth of fibroblasts and keratinocytes, we established short-term, primary cultures of melanocytes from skin biopsies of common acquired nevi, dysplastic nevi, and from metastatic melanoma. Using PCR-single-stranded conformational...
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