Article
Specificity of DNA binding activity of the Bacillus subtilis catabolite control protein CcpA.
Journal of bacteriology - 1 Sept 1995
Kim J H, Guvener Z T, Cho J Y, Chung K C, Chambliss G H
Abstract excerpt
CcpA was purified from Escherichia coli BL21 (lambda DE3)/pLysS carrying plasmid pTSC5, which was constructed by inserting the ccpA gene into the polycloning site of pGEM4. The purified protein migrated in sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an apparent mass of 38 kDa b...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Base Sequence
- Blotting, Western
- DNA, Bacterial
- DNA-Binding Proteins
- Deoxyribonuclease I
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data
- Mutation
- Protein Binding
- Recombinant Proteins
- Repressor Proteins
- alpha-Amylases